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The localisation of fluorophores is an important aspect of the determinationof the biological function of cellular systems. Quantum correlation microscopy(QCM) is a promising technique for providing diffraction unlimited emitterlocalisation that can be used with either confocal or widefield modalities.However, so far, QCM has not been applied to three dimensional localisationproblems. Here we show that quantum correlation microscopy providesdiffraction-unlimited three-dimensional localisation for two emitters within asingle diffraction-limited spot. By introducing a two-stage maximum likelihoodestimator, our modelling shows that localisation precision scales as$1/\sqrt{t}$ where $t$ is the total detection time. Diffraction unlimitedlocalisation is achieved using both intensity and photon correlation fromHanbury Brown and Twiss measurements at as few as four measurement locations.

Localising two sub-diffraction emitters in 3D using quantum correlation microscopy