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Reliable and consistent preparation of atom probe tomography (APT) specimensfrom aqueous and hydrated biological specimens remains a significant challenge.One particularly difficult process step is the use of a focused ion beam (FIB)instrument for preparing the required needle-shaped specimen, typicallyinvolving a "lift-out" procedure of a small sample of material. Here, twoalternative substrate designs are introduced that enable using FIB only forsharpening, along with example APT datasets. The first design is a laser-cutFIB-style half-grid close to those used for transmission-electron microscopy,that can be used in a grid holder compatible with APT pucks. The second designis a larger, standalone self-supporting substrate called a "crown", withseveral specimen positions that self-aligns in APT pucks, prepared byelectrical discharge machining (EDM). Both designs are made nanoporous, toprovide strength to the liquid-substrate interface, using chemical and vacuumdealloying. We select alpha brass a simple, widely available, lower-costalternative to previously proposed substrates. We present the resultingdesigns, APT data, and provide suggestions to help drive wider communityadoption.

Scalable Substrate Development for Aqueous Biological Samples for Atom Probe Tomography