English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Polycyclic aromatic hydrocarbons (PAHs) are highly toxic, carcinogenicsubstances. On soils contaminated with PAHs, crop cultivation, animal husbandryand even the survival of microflora in the soil are greatly perturbed,depending on the degree of contamination. Most microorganisms cannot toleratePAH-contaminated soils, however, some microbial strains can adapt to theseharsh conditions and survive on contaminated soils. Analysis of the metagenomesof contaminated environmental samples may lead to discovery of PAH-degradingenzymes suitable for green biotechnology methodologies ranging frombiocatalysis to pollution control. In the present study, our goal was to applya metagenomic data search to identify efficient novel enzymes in remediation ofPAH-contaminated soils. The metagenomic hits were further analyzed using a setof bioinformatics tools to select protein sequences predicted to encodewell-folded soluble enzymes. Three novel enzymes (two dioxygenases and oneperoxidase) were cloned and used in soil remediation microcosms experiments.The novel enzymes were found to be efficient for degradation of naphthalene andphenanthrene. Adding the inorganic oxidant CaO2 further increased the degradingpotential of the novel enzymes for anthracene and pyrene. We conclude thatmetagenome mining paired with bioinformatic predictions, structural modellingand functional assays constitutes a powerful approach towards novel enzymes forsoil remediation.

Novel enzymes for biodegradation of polycyclic aromatic hydrocarbons: metagenomics-linked identification followed by functional analysis