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The objective of the current study was to assess the effect of dietarysupplementations of mannan-oligosaccharide, live yeast, and a combination ofthese two additives on growth performance, histo-morphology of the rumen, andmuscle morphometric attributes in buffalo calves. A total of twenty buffalocalves (average weight of 25 kg) having 3 months of age were distributedaccording to a complete randomized design. All animals were individuallystalled in the shed and were fed ad-libitum. Experimental animals were dividedinto four groups for 67 days: Control group(without the inclusion of dietarysupplementation); MOS group (Mannan oligosaccharide 5 g/clave/day; Yeast group(Live yeast 2g/calve/day) and Mixed group (MOS + Live Yeast 2.5g + 1g)/calve/day. Experimental results revealed that combined supplementation of MOSand Yeast and MOS alone resulted in an increased number of short-chain fattyacids in the rumen as well as ruminal pH (P<0.05). Results showed a significantimprovement in average daily gain and FCR of MOS and Mixed supplemented groups(P<0.05). Histomorphological evaluation of rumen mucosal epithelium showed asignificant improvement in the mixed-supplemented group (P<0.05) as compared tothe yeast-supplemented and control groups. Muscle quality parameters such asmeat texture showed significant improvement in MOS and mix-supplemented groups.Histological examination of longissimus dorsi muscle cross-section showed asignificantly higher(P<0.05) muscle fiber and muscle fascicle diameter in bothMOS and mix-supplemented calves groups. In conclusion, the results of thisexperiment revealed that the dietary addition of MOS, Live yeast, and theircombination have positive effects on growth performance, rumen histologyindices, and muscle morphometric features in buffalo calves.

Efficacy of mannan-oligosaccharide and live yeast feed additives on  performance, rumen morphology, serum biochemical parameters and muscle  morphometric characteristics in buffalo calves