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Hydrophobic domains provide specific microenvironment for essentialfunctional activities in life. Herein, we studied how the coassembling ofpeptides with catecholamines regulate the hydrophobic domain-containingnanostructures for fluorescence enhancement. By peptide encoding andcoassembling with catecholamines of different hydrophilicities, a series ofhierarchical assembling systems were constructed. In combination with moleculardynamics simulation, we experimentally discovered the hydrophobic domain ofchromophore microenvironment regulates the fluorescence of coassemblednanostructures. Our results shed light on the rational design of fluorescentbio-coassembled nanoprobes for biomedical applications.

Regulating the Hydrophobic Domain in Peptide-Catecholamine Coassembled Nanostructures for Fluorescence Enhancement