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We developed pgMAP, an analysis pipeline to map gRNA sequencing reads fromdual-targeting CRISPR screens. pgMAP output includes a dual gRNA read countstable and quality control metrics including the proportion of correctly-pairedreads and CRISPR library sequencing coverage across all time points andsamples. pgMAP is implemented using Snakemake and is available open-sourceunder the MIT license at https://github.com/fredhutch/pgmap_pipeline.

pgMAP: a pipeline to enable guide RNA read mapping from dual-targeting CRISPR screens