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Reconstituted cytoskeleton composites have emerged as a valuable model systemfor studying non equilibrium soft matter. Faithful capture of the dynamics ofthese three dimensional, dense networks calls for optical sectioning oftenassociated with fluorescence confocal microscopes. However, recent developmentsin light sheet microscopy has established it as a cost effective, and at timessuperior, alternative. To make the LSM accessible to cytoskeleton researchersless familiar with optics, we present a step by step beginners guide tobuilding a versatile light sheet microscope from off the shelf components. Toenable sample mounting with traditional slide samples, our LSM follows thesingle objective light sheet design, which utilizes a single objective for bothexcitation and emission collection. We describe the function of each componentof the SOLS in sufficient detail to allow readers to modify the instrumentationand design to fit their specific needs. Finally, we demonstrate the use of ourcustom SOLS instrument by visualizing asters in kinesin driven microtubulenetworks.

Design and Build of a Customizable Light Sheet for Visualization of Cytoskeleton Networks