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Aberrations and multiple scattering in biological tissues critically distortlight beams into highly complex speckle patterns. In this regard, digitaloptical phase conjugation (DOPC) is a promising technique enabling in-depthfocusing. However, DOPC becomes challenging when using fluorescent guide-starsfor four main reasons: The low photon budget available, the large spectralbandwidth of the fluorescent signal, the Stokes shift between the emission andthe excitation wavelength, and the absence of reference beam preventingholographic measurement. Here, we demonstrate the possibility to focus a laserbeam through multiple-scattering samples by measuring speckle fields in asingle acquisition step with a reference-free, high-resolution wavefrontsensor. By taking advantage of the large spectral bandwidth of forward multiplyscattering samples, Digital Fluorescence Phase Conjugation (DFPC) is achievedto focus a laser beam at the excitation wavelength while measuring thebroadband speckle field arising from a micron-sized fluorescent bead.

Single-shot Digital Optical Fluorescence Phase Conjugation Through Forward Multiply Scattering Samples