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Fundamental properties of light unavoidably impose features on imagescollected using fluorescence microscopes. Modeling these features is ever moreimportant in quantitatively interpreting microscopy images collected at scaleson par or smaller than light's wavelength. Here we review the opticsresponsible for generating fluorescent images, fluorophore properties,microscopy modalities leveraging properties of both light and fluorophores, inaddition to the necessarily probabilistic modeling tools imposed by thestochastic nature of light and measurement.

Fluorescence Microscopy: a statistics-optics perspective