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Flow cytometry is a widespread and powerful technique, whose resolution isdetermined by its capacity to accurately distinguish fluorescently positivepopulations from negative ones. However, most informative results are discardedwhile performing the measurements of conventional flow cytometry, e.g., thecell size, shape, morphology, and distribution or location of labeled exosomeswithin the unpurified biological samples. We, herein, propose a novel approachusing an anti-diffraction light sheet with anisotroic feature to excitefluorescent tags. Constituted by an anti-diffraction Bessel-Gaussian beamarray, the light sheet is 12 $\mu$m wide, 12 $\mu$m high, with a thickness of$~ 0.8 \mu$m. The intensity profile of the excited fluorescent signal can,therefore, reflect the size and allow samples in the range from O(100 nm) to 10$\mu$m (e.g., blood cells) to be transported via hydrodynamic focusing in amicrofluidic chip. The sampling rate is 500 kHz provides a capability of highthroughput without sacrificing the spatial resolution. Consequently, theproposed anti-diffraction light-sheet flow cytometry (ADLSFC) can obtain moreinformative results than the conventional methodologies, and is able to providemultiple characteristics (e.g., the size and distribution of fluorescentsignal) helping to distinguish the target samples from the complex backgrounds.

Flow cytometry with anti-diffraction light sheet (ADLS) by spatial light modulation