English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Cyanobacteriochromes are bi-stable photoreceptor proteins with desirablephotochemical properties for biotechnological applications such as optogeneticsor fluorescence microscopy. Here, we investigated Slr1393-g3, acyanobacteriochrome that reversibly photo-switches between a red-absorbing (Pr)and green-absorbing (Pg) form. We applied advanced IR spectroscopic methods totrack the sequence of intermediates during the photocycle over many orders inmagnitude in time. In the conversion from Pg to Pr, we have revealed a newintermediate which precedes the Pr formation by using transient IRspectroscopy. In addition, stationary and transient 2D~IR experiments measuredthe vibrational couplings between different groups of the chromophore and theprotein during these intermediate states. Anharmonic QM/MM calculations predictspectra in close-to-quantitative agreement with experimental 2D~IR spectra ofthe initial and the final state of the photocycle. They facilitate theassignment of the IR spectra and provide an atomistic insight into the couplingmechanism. This serves as a basis for the interpretation of the spectroscopicresults and suggests structural changes of the intermediates along thephotocycle.

Transient 2D IR spectroscopy and multiscale simulations reveal vibrational couplings in the Cyanobacteriochrome Slr1393-g3