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During In Vitro Fertilisation (IVF), combining time-lapse contrast microscopywith suitable image processing techniques could facilitate non-invasiveclassification of oocyte health, driving oocyte selection to maximise successrates. One image processing technique, Differential Dynamic Microscopy (DDM),has been used in a variety of microscopy settings including bright field, darkfield and fluorescence. However, in some applications, effects stemming fromthe choice of microscopy may invalidate underlying assumptions of DDM. Here, westudy the DDM analysis of differential interference contrast (DIC) microscopymovies. DIC exhibits a characteristic shadowing effect that gives the illusionof a 3D appearance, which we show causes deformation of the output DDM matrix.We present a mathematical description of this deformation, and conclude that,when studying isotropic motion, no account of the DIC shadow needs to beconsidered during DDM analysis. We validate our conclusions with simulatedparticle data, and with DIC images of colloidal dispersions. Although thisconclusion does not generally extend to anisotropic motion, we demonstrate thatfor directed advection and diffusion behaviour, parameter fitting invariancestill holds. These results validate the current practice of applying DDM toDIC, and are a foundation for further exploration of DDM in otherphase-contrast image datasets.

Differential Dynamic Microscopy can be applied to Differential Interference Contrast images despite shadowing effects