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We demonstrate label-free drug response evaluations of human breast (MCF-7)and colon (HT-29) cancer spheroids via dynamic optical coherence tomography(OCT). The MCF-7 and HT-29 spheroids were treated with paclitaxel (PTX, orTaxol) and the active metabolite of irinotecan (SN-38), respectively. The drugswere applied using 0 (control), 0.1, 1, and 10 uM concentrations with treatmenttimes of 1, 3, and 6 days. The samples were scanned using a repeated rasterscan protocol and two dynamic OCT algorithms, logarithmic intensity variance(LIV) and late OCT correlation decay speed (OCDSl) analyses, were applied tovisualize the tissue and cellular dynamics. Different drug response patterns ofthe two spheroid types were visualized clearly and analyzed quantitatively byLIV and OCDSl imaging. For both spheroid types, structural corruptions andreduction of LIV and OCDSl were observed. These results may indicate differentmechanisms of the drug action. The results suggest that dynamic OCT can be usedto highlight drug response patterns and perform anti-cancer drug testing.

Label-free drug response evaluation of human derived tumor spheroids using three-dimensional dynamic optical coherence tomography