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Fourier light-field microscopy (FLFM) uses a micro-lens array (MLA) tosegment the Fourier Plane of the microscopic objective lens to generatemultiple two-dimensional perspective views, thereby reconstructing thethree-dimensional(3D) structure of the sample using 3D deconvolutioncalculation without scanning. However, the resolution of FLFM is still limitedby diffraction, and furthermore, dependent on the aperture division. In orderto improve its resolution, a Super-resolution optical fluctuation Fourier lightfield microscopy (SOFFLFM) was proposed here, in which the Sofi method withability of super-resolution was introduced into FLFM. SOFFLFM uses higher-ordercumulants statistical analysis on an image sequence collected by FLFM, and thencarries out 3D deconvolution calculation to reconstruct the 3D structure of thesample. Theoretical basis of SOFFLFM on improving resolution was explained andthen verified with simulations. Simulation results demonstrated that SOFFLFMimproved lateral and axial resolution by more than sqrt(2) and 2 times in the2nd and 4th order accumulations, compared with that of FLFM.

SOFFLFM: Super-resolution optical fluctuation Fourier light-field microscopy