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Modern research in the life sciences is unthinkable without computationalmethods for extracting, quantifying and visualizing information derived frombiological microscopy imaging data. In the past decade, we observed a dramaticincrease in available software packages for these purposes. As it isincreasingly difficult to keep track of the number of available image analysisplatforms, tool collections, components and emerging technologies, we provide aconservative overview of software we use in daily routine and give insightsinto emerging new tools. We give guidance on which aspects to consider whenchoosing the right platform, including aspects such as image data type, skillsof the team, infrastructure and community at the institute and availability oftime and budget.

A Hitchhiker`s Guide through the Bio-image Analysis Software Universe