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Acoustical tweezers based on focused acoustical vortices open some tremendousperspectives for the in vitro and in vivo remote manipulation of millimetricdown to micrometric objects, with combined selectivity and applied forces outof reach with any other contactless manipulation technique. Yet, the synthesisof ultra-high frequency acoustical vortices to manipulate precisely micrometricobjects remains a major challenge. In this paper, the synthesis of a 250 MHzacoustical vortex is achieved with an active holographic source based onspiraling interdigitated transducers. It is shown that this ultra-highfrequency vortex enables to trap and position individual particles in astandard microscopy environment with high spatial selectivity and nanoNewtonforces. This work opens perspectives to explore acoustic force spectroscopy insome force ranges that were not accessible before.

Ultra-high frequency vortex-based tweezers for microparticles manipulation with high spatial selectivity and nanoNewton forces