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This study included isolate petroleum hydrocarbons degradable bacteria anddevelops a consortium or a mixture of bacteria with high biodegradationcapabilities which can be used in biological treatment units of thecontaminated soils before release. In this studyt ten bacterial strains wereisolated from soils contaminated with crude oil, by primary and secondaryscreening, and by using the sterile saline solution with 1% of crude oil, fivebacterial isolates that can degrade oil were identified. The bacterial isolateswere isolated from polluted soils with crude oil, the samples were collectedfrom different areas of the Baiji refinery, and samples of heavy crude oilextracted from Qayyarah fields were used in designing the biodegradationexperiments, the five isolates were diagnosed based on phenotypic, culture andbiochemical characterizes, and detection of the gene sequence of bacterialisolates (16SrRNA). The (16SrRNA) gene sequence of the bacterial isolates wasrecorded under the accession numbers LC596402, LC596403, LC596406, LC596404,LC596405, for the genus (AM-I-1, AM-I-2, AM-I-5) and the species (AM-I-3,AM-I-4) that following Bacillus Sp respectively, in the NCBI's GenBank, theefficiency of the five isolates were examined for utilizing petroleumhydrocarbons using a sterile mineral salt medium MS supplemented with crude oilas the sole source of carbon with different concentrations, the results showedthat the decomposition of petroleum hydrocarbons at the concentrations (0.5, 1,1.5, 2, 3) % reached (57.32, 69.36,63.71, 75.20, 68.60) %, respectively, formixed isolates, depending on the results of the gas chromatography (GC)analysis.

Bioremediation of Contaminated Soil with Crude Oil Using Consortium of bacteria