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The mechanism and driving forces of chromosome segregation in the bacterialcell cycle of E. coli is one of the least understood events in its life cycle.Using principles of entropic repulsion between polymer loops confined in acylinder, we use Monte carlo simulations to show that the segregation dynamicsis spontaneously enhanced by the adoption of a certain DNA-polymer architectureas replication progresses. Secondly, the chosen polymer-topology ensures itsself-organization along the cell axis while segregation is in progress, suchthat various chromosomal loci get spatially localized. The time evolution ofloci positions quantitatively match the corresponding experimentally reportedresults, including observation of the cohesion time and the ter-transition.Additionally, the contact map generated using our bead-spring model reproducesthe four macro-domains of the experimental Hi-C maps. Lastly, the proposedmechanism reproduces the observed universal dynamics as the sister lociseparate during segregation.  It was already hypothesized and expected that SMC proteins, e.g. MukBEFcontribute over and above entropic repulsion between bacterial-DNAring-polymers to aid the segregation of daughter DNAs in the E.coli cell cycle.We propose that cross-links (plausibly induced by SMC proteins) at crucialpositions along the contour is enough to provide sufficient forces forsegregation within reasonable time scales. A mapping between Monte Carlodiffusive dynamics time scales and real time units helps us use experimentallyrelevant numbers for our modeling.

DNA-polymer architecture orchestrates the segregation and spatio-temporal organization of E. coli chromosomes during replication in slow growth