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Spectroscopic stimulated Raman scattering (SRS) imaging has become a usefultool finding a broad range of applications. Yet, wider adoption is hindered bythe bulky and environmentally-sensitive solid-state optical parametricoscillator (OPO) in current SRS microscope. Moreover, chemically-informativemulti-window SRS imaging across C-H, C-D and fingerprint Raman regions ischallenging due to the slow wavelength tuning speed of the solid-state OPO. Inthis work, we present a multi-window SRS imaging system based on a compact androbust fiber laser with rapid and widely tuning capability. To address therelative intensity noise intrinsic to fiber laser, we implemented auto-balanceddetection which enhances the signal-to-noise ratio of stimulated Raman lossimaging by 23 times. We demonstrate high-quality SRS metabolic imaging offungi, cancer cells, and Caenorhabditis elegans across the C-H, C-D andfingerprint Raman windows. Our re-sults showcase the potential of the compactmulti-window SRS system for a broad range of applications.

Multi-window SRS imaging using a rapid widely tunable fiber laser