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Identifying, visualising and ultimately tracking dynamically movingnon-fluorescent nanoparticles in the presence of non-specific scattering is along-standing challenge across the nano- and life-sciences. In this work wedemonstrate that our recently developed ultrafast holographic transient (UHT)microscope is ideally suited for meeting this challenge. We show that UHTmicroscopy allows reliably distinguishing off-resonant, dielectric, fromresonant, metallic, nanoparticles, based on the phototransient signal: apre-requisite for single-particle tracking in scattering environments. We thendemonstrate the capability of UHT microscopy to holographically localize in 3Dsingle particles over large volumes of view. Ultimately, we combine the twoconcepts to simultaneously track several tens of freely diffusing goldnanoparticles, within a 110x110x110 $\mu$m volume of view at an integrationtime of 10 ms per frame, while simultaneously recording their phototransientsignals. The combined experimental concepts outlined and validated in this worklay the foundation for background-free 3D single-particle tracking applicationsor spectroscopy in scattering environments and are immediately applicable tosystems as diverse as live cells and tissues or supported heterogeneouscatalysts.

Widefield phototransient imaging for visualizing 3D motion of resonant particles in scattering environments