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Nitrogen-vacancy (NV) color centers in diamond have been demonstrated asuseful magnetic sensors, in particular for measuring spin fluctuations,achieving high sensitivity and spatial resolution. These abilities can be usedto explore various biological and chemical processes, catalyzed by ReactiveOxygen Species (ROS). Here we demonstrate a novel approach to measure andquantify Hydroxyl radicals with high spatial resolution, using the fluorescencedifference between NV charged states. According to the results, the achieved NVsensitivity is $11 \pm 4 \frac{nM}{\sqrt Hz}$, realized in-situ without spinlabels and localized to a volume of $\sim 10$ picoliter.

High-Sensitivity, High-Resolution Detection of Reactive Oxygen-Species Concentration Using NV Centers