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Holographic microscopy combined with forward modeling and inference allowscolloidal particles to be characterized and tracked in three dimensions withhigh precision. However, current models ignore the effects of opticalaberrations on hologram formation. We investigate the effects of sphericalaberration on the structure of single-particle holograms and on the accuracy ofparticle characterization. We find that in a typical experimental setup,spherical aberration can result in systematic shifts of about 2% in theinferred refractive index and radius. We show that fitting with a model thataccounts for spherical aberration decreases this aberration-dependent error bya factor of two or more, even when the level of spherical aberration in theoptical train is unknown. With the new generative model, the inferredparameters are consistent across different levels of aberration, makingparticle characterization more robust.

Improving holographic particle characterization by modeling spherical aberration