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We compute the overlap Dirac spectrum on three ensembles generated using 2+1flavor domain wall fermions. The spectral density is determined up to$\lambda\sim$100 MeV with sub-percentage statistical uncertainty. The threeensembles have different lattice spacings and two of them have quark massestuned to the physical point. We show that we can resolve the flavor content ofthe sea quarks and constrain their masses using the Dirac spectral density. Wefind that the density is close to a constant below $\lambda\le$ 20 MeV (but10\% higher than that in the 2-flavor chiral limit) as predicted by chiralperturbative theory ($\chi$PT), and then increases linearly due to the strangequark mass. Using the next to leading order $\chi$PT, one can extract the lightand strange quark masses with $\sim$20\% uncertainties. Using thenon-perturbative RI/MOM renormalization, we obtain the chiral condensates at$\overline{\textrm{MS}}$ 2 GeV as $\Sigma=(260.3(0.7)(1.3)(0.7)(0.8)\\textrm{MeV})^3$ in the $N_f=2$ (keeping the strange quark mass at the physicalpoint) chiral limit and $\Sigma_0=(232.6(0.9)(1.2)(0.7)(0.8)\ \textrm{MeV})^3$in the $N_f=3$ chiral limit, where the four uncertainties come from thestatistical fluctuation, renormalization constant, continuum extrapolation andlattice spacing determination. Note that {$\Sigma/\Sigma_0=1.40(2)(2)$ is muchlarger than 1} due to the strange quark mass effect.

Detecting flavor content of the vacuum using the Dirac operator spectrum