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Inverse spectroscopic optical coherence tomography (IS-OCT) methods applyinverse problem formulations to acquired spectra to estimate depth-resolvedsample properties. In the current study, we modelled thetime-frequency-distributions using Lambert-Beer law and implemented IS-OCTusing backscattering spectra calculated from Mie theory, then demonstrated thealgorithm on polystyrene microspheres under idealized conditions. The resultsare significant because the method generates depth dependent estimates of boththe concentration and diameter of scattering particles.

Inverse spectroscopic optical coherence tomography (ISOCT) for characterization of particle size and concentration