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We developed a multifocus optical-resolution photoacoustic microscope (MPAM)with extended depth of field (DoF) by using a tunable acoustic gradient (TAG)lens and a pulse delay method. TAG lens is designed to control the divergenceangle of the laser beam. Three fibers with different length are used to outputthree laser pulses with different delay time. By controlling the fire time ofthe laser pulses, we can get three focal spot through TAG lens in our system inone A-line data acquisition. The DoF of MPAM measured using a vertically tiltedcarbon fiber is about 775 micrometers. The lateral resolution is estimated tobe 2.8 micrometers. A phantom consists of tungsten wires and a zebrafish isused to verify the large depth-of-field of our system.

Parallel multifocus optical-resolution photoacoustic microscopy based on tunable acoustic gradient lens and fiber delay network