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Vortex is a universal and significant phenomenon that has been known forcenturies. However, creating vortices to the atomic limit has remained elusivebecause that the characteristic length to support a vortex is usually muchlarger than the atomic scale. Very recently, it was demonstrated thatintervalley scattering induced by the single carbon defect of graphene leads tophase winding over a closed path surrounding the defect. Motivated by this, wedemonstrate, in this Letter, that the single carbon defects at A and Bsublattices of graphene can be regarded as pseudospin-mediated atomic-scalevortices with angular momenta l = +2 and -2, respectively. The quantuminterferences measurements of the interacting vortices indicate that thevortices cancel each other, resulting in zero total angular momentum, in the|A| = |B| case, and they show aggregate chirality and angular momenta similarto a single vortex of the majority in the |A| not equal to |B| case, where |A|(|B|) is the number of vortices with angular momenta l = +2 (l = -2).

Pseudospin-mediated Atomic-scale Vortices and Their Quantum Interferences in Monolayer Graphene