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{\mu}Manager, an open-source microscopy acquisition software, has been anessential tool for many microscopy experiments over the past 15 years, but isnot easy to use for experiments in which image acquisition and analysis areclosely coupled. This is because {\mu}Manager libraries are written in C++ andJava, whereas image processing is increasingly carried out with data scienceand machine learning tools most easily accessible through the Pythonprogramming language. We present Pycro-Manager, a tool that enables rapiddevelopment of such experiments, while also providing access to the wealth ofexisting tools within {\mu}Manager through Python.

Pycro-manager: open-source software for integrated microscopy hardware control and image processing