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Advanced characterisation techniques were used on LPBF Ti-6Al-4V samplesproduced on a heated base plate. When the substrate temperature is 100{\deg}Cthe elongation is 6\%, which increases and peaks at 10\% at 570{\deg}C, thensharply decreases to zero ductility at 770{\deg}C. At 100{\deg}C, a heavilystrained and twinned microstructure, primarily composed of {\alpha}+{\alpha}',was observed and it was comparable to asbuilt microstructures obtained byconventional LPBF methods. At higher temperatures, twins are no longer presentand instead nano-scale {\beta} precipitates are observed within {\alpha}' and{\alpha}, as well as dislocation networks (570{\deg}C) and tangles(770{\deg}C). Solute segregation at crystal defects was observed in allpre-heating conditions. Al and V segregation at microtwins was observed in the100{\deg}C sample, reporting for the first time `selective' and mutuallyexclusive Al- and V-rich regions forming in adjacent twins. V segregation atdislocations was observed in the 570{\deg}C and 770{\deg}C samples, consistentwith the higher preheating temperatures. High O contents were measured in allsamples but with apparent opposing effects. At 100{\deg}C and 570{\deg}C wasestimated to be below the critical threshold for O embrittlement and locallyaids in maintaining a strength high by solid solution strengthening, whereas at770{\deg}C it was above the threshold, therefore failing in a brittle fashion.Based on these observations, the initial increase in ductility from 100{\deg}Cto 570{\deg}C is attributed to a reduction in microtwins and the dislocationnetworks acting as `soft barriers' for slip within a coarser microstructure.The lack of ductility at 770{\deg}C was attributed to local soluteredistribution causing dislocation pinning and an increase of O content in thissample.

Effect of bed temperature on solute segregation and mechanical properties in Ti-6Al-4V produced by selective laser melting