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In the high-throughput screening (HTS) campaigns, the Z'-factor and strictlystandardized mean difference (SSMD) are commonly used to assess the quality ofassays and to select hits. However, these measures are vulnerable to outliersand their performances are highly sensitive to background distributions. Here,we propose an alternative measure for assay quality assessment and hitselection. The proposed method is a non-parametric generalized variant of SSMD(GSSMD). In this paper, we have shown that the proposed method provides morerobust and intuitive way of assay quality assessment and hit selection.

GSSMD: New metric for robust and interpretable assay quality assessment and hit selection