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We collaborated with Amgen and spent five years in designing and fabricatingnext generation multi-well plate imagers based on Fourier ptychographicmicroscopy (FPM). A 6-well imager (Emsight) and a low-cost parallel microscopicsystem (96 Eyes) based on parallel FPM were reported in our previous work.However, the effect of liquid meniscus on the image quality is much strongerthan anticipated, introducing obvious wavevector misalignment and additionalimage aberration. To this end, an adaptive wavevector correction (AWC-FPM)algorithm and a pupil recovery improvement strategy are presented to solvethese challenges in situ. In addition, dual-channel fluorescence excitation isadded to obtain structural information for microbiologists. Experiments aredemonstrated to verify their performances. The accuracy of angular resolutionwith our algorithm is within 0.003 rad. Our algorithms would make the FPMalgorithm more robust and practical and can be extended to other FPM-basedapplications to overcome similar challenges.

In situ correction of liquid meniscus in cell culture imaging system based on parallel Fourier ptychographic microscopy (96 Eyes)