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The main object of this investigation was to shed light on the information onthe level of Cu uptake by Pseudomonas aeruginosa that has been previouslyisolated from patients using Gram-negative E. aerogenes ans a Gram-positiveBacillus thuringiensis as a control for Cu uptake measurements. Cu uptake datashowed that maximum Cu uptake was obtained by the Pseudomonas aeruginosa. Themetal uptake was dependent on the type of biosorbent with differentaccumulation affinities toward the tested chemical form of the copper. Cellsharvested at exponential growth phase showed slightly higher Cu uptake than atstationary phase, which reflect that the Cu uptake is metabolismdependent-process. The increasing order of affinity of the cupric chloride andcupric sulphate towards the three genera were almost constant. However, wherecupric nitrate was used, the copper uptake behavior in Pseudomonas aeroginosawas not changed from those results with the other two chemical forms, whereaswith E. aerogenes & B. thuringiensis, different Cu uptake behaviors wereobserved. In B. thuengenesis, the copper uptake rate as a function of initialCu concentration were shown to increase with increasing the initialconcentration of Cu, constantly above 320 ppm as compared with others. InEnterobacter with cupric nitrate, different bahavior was also shown in whichthe copper uptake decline sharply beyond the 320 ppm. These results reflectthat the Cu uptake in P. aeruginosa is with different mechanisms as comparedwith other tested bacteria.

Effect of Copper Chemical Form on The Growth of Pseudomonas aeruginosa Isolated from Burned Patients and on Its Cu Uptake