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Antibactrial activity of Asteriscus graveolens methanolic extract and itssynergy effect with fungal mediated silver nanoparticles (AgNPs) against someenteric bacterial human pathogen was conducted. Silver nanoparticles weresynthesized by the fungal strain namely Tritirachium oryzae W5H as reportedearly. In this study, MICs of AgNPs against E. aerogenes, Salmonella sp., E.coli and C. albicans in order were 2.13, 19.15, 0.08 and 6.38 micrograms permL, respectively, while the MICs of A. graveolens ethanolic extract against thesame bacteria were 4, 366, 3300 and 40 micrograms per mL, respectively. The MICvalues at concentration less than 19.15 and 40 micrograms per mL indicating thepotent bacteriostatic effect of AgNPs and A. graveolens ethanolicextract.Increasing in IFA was reported when Nitrofurantion and Trimethoprimwere combined with Etoh extract with maximum increase in IFA by 6 and 12 foldsfor, respectively. Also, 10 folds increasing in IFA was reported whentrimethoprim was combined with AgNPs: Etoh extract.But, there were nosynergistic effect between the antifungal agents (Caspofungin and Micafungin)combined with AgNPs and or A. graveolens ethanolic extract against C.albicans.The potent synergistic effect of A. graveolens ethanolic extractand/or NPs with the conventional antibiotics is novel in inhibiting antibioticsresistant bacteria. In this study, remarkable increasing in the antibacterialactivity, when the most resistant antibiotics combined with A. graveolensethanolic extract and/or NPs was reported.

Antibactrial Activity of Asteriscus graveolens Methanolic Extract:  Synergistic Effect with Fungal Mediated Nanoparticles against Some Enteric  Bacterial Human Pathogens