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Motile human-skin fibroblasts form macroscopic swirling patterns when grownto confluence on a culture dish. In this paper, we investigate the effect ofcoating the culture-dish surface with collagen on the resulting pattern, usinghuman-skin fibroblast NB1RGB cells as the model system. The presence of thecollagen coating is expected to enhance the adherence of the fibroblasts to thedish surface, and thereby also enhance the traction that the fibroblasts haveas they move. We find that, contrary to our initial expectation, the coatingdoes not significantly affect the motility of the fibroblasts. Their eventualnumber density at confluence is also unaffected. However, the coherence lengthof cell orientation in the swirling pattern is diminished. We also find thatthe fibroblasts cultured in a collagen-coated dish are rounder in shape andshorter in perimeter, compared to those cultured in an uncoated dish. Wehypothesize that the rounder cell-shape which weakens the cell-cell nematiccontact interaction is responsible for the change in coherence length. A simplemathematical model of the migrating fibroblasts is constructed, whichdemonstrates that constant motility with weaker nematic interaction strengthdoes indeed lead to the shortening of the coherence length.

The effects of coating culture dishes with collagen on fibroblast cell shape and swirling pattern formation