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The total phenols and flavonoids content of different polar solvent extractsfrom Aurea Helianthus flowers, and their antioxidant activity were determined.The ethanol extract of Aurea Helianthus flowers were suspended in water andfractionated using different polar solvents; hexane, chloroform, ethyl acetate,butanol and water. The parameters of each extract mentioned above weredetermined using Floin-ciocalteu reagent(FCR) method, AlCl3 colorimetry method,ferric reducing ability of plasma(FRAP) assay, total antioxidant activity(TAA)assay and DPPH radical scavenging assay. The highest total phenolscontent(516.21 mg GAE/g) and flavonoids content(326.06 mg QCE/g) were obtainedin ethyl acetate extract, the correlation between TPC and TFC assay was foundedto be 0.967. All polar solvent extracts of Aurea Helianthus flowers showedsignificant antioxidant effects, the hightest inhibition was obtained in ethylacetate and choroform extracts and the lowest inhibition in the water extract.There is a good correlation of total phenols and flavonoids content withantioxidant activity. This work indicated that the polar solvent extracts ofAurea Helianthus flowers contain high phenols and flavonoids content andexhibited antioxidant activities in vitro, therefore, could be candidates foruse as natural antioxidant.

Effect of different polarity solvents on total phenols and flavonoids  content, and In-vitro antioxidant properties of flowers extract from Aurea  Helianthus