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Live time-lapse microscopy is essential for a wide range of biologicalapplications. Software-based automation is the gold standard for the operationof hardware accessories necessary for image acquisition. Given that currentsoftware packages are neither affordable nor open to complex structuredprogramming, we have developed a Matlab-based graphical user interface (GUI)that is fundamentally accessible while providing limitless avenues for furthercustomization. The GUI simultaneously communicates with the open-source,cross-functional platform micro-Manager for controlling hardware for time-lapseimage acquisition and with other software for image processing. The use of theGUI is demonstrated through an 18-hour cell migration experiment. The resultssuggest that the GUI can generate high-quality, high-throughput time-lapseimages. The core code behind the GUI is open-source so that it can be modifiedand upgraded. Therefore, it benefits the researchers worldwide by providingthem a fundamental yet functional template to add in advanced features specificto their needs, such as additional non-microscopy hardware parts and softwarepackages supported by the Matlab software.

Open-Source Matlab-Based Graphical User Interface (GUI) For Computer Control of Microscopes Using Micro-Manager