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Variously stained histology slices are routinely used by pathologists toassess extracted tissue samples from various anatomical sites and determine thepresence or extent of a disease. Evaluation of sequential slides is expected toenable a better understanding of the spatial arrangement and growth patterns ofcells and vessels. In this paper we present a practical two-step approach basedon diffeomorphic registration to align digitized sequential histopathologystained slides to each other, starting with an initial affine step followed bythe estimation of a detailed deformation field.

Accurate and Robust Alignment of Variable-stained Histologic Images Using a General-purpose Greedy Diffeomorphic Registration Tool