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In this work, wild-type and heterozygous \b{eta}-thalassaemic mice wereenriched with 57Fe through gastrointestinal absorption to characterize in moredetails the iron complexes appeared in the measured Mossbauer spectra. The 57Feenrichment method was validated and Mossbauer spectra were obtained at 80K fromblood samples from wild-type and \b{eta}-thalassaemic mice at 1, 3, 6, and 9months of age. As expected, the haemoglobin levels of the thalassaemic micewere lower than from normal mice indicating anaemia. Furthermore, significantamounts of ferritin-like iron were observed in the thalassaemic mice samples,which decreased with mouse age, reflecting the pattern of reticulocyte countreduction reported in the literature.

57Fe enrichment in mice for \b{eta}-thalassaemia studies via Mossbauer spectroscopy of blood samples