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In this paper, we investigated if the differences consistently noted insurvival and plantlet production between cryopreserved and non-cryopreserved,control sugarcane embryogenic calluses were related to modifications inducedduring cryopreservation in the structural and functional integrity of cellmembranes. For this, the evolution of electrolyte leakage, lipid peroxidationproducts and cell membrane protein contents was measured during 5 d aftercryopreservation. Differences between control and frozen calluses were observedonly during the first 2 (electrolyte leakage) or 3 d (lipid peroxidationproducts and membrane protein content) after freezing. It was not possible tolink these differences with the differences noted in survival and plantproduction between control and cryopreserved calluses. Additional studies arethus needed to elucidate which biochemical factors, linked to survival andplantlet regeneration, are affected by cryopreservation.

Effect of cryopreservation on the structural and functional integrity of cell membranes of sugarcane (Saccharum sp.) embryogenic calluses