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Optogenetics is a rapidly growing field of biotechnology, potentiallyallowing a deeper understanding and control of complex biological networks. Themajor challenge is the multiplexed control of several optogenetic components inthe presence of significant spectral cross talk. We propose and demonstratethrough simulations a new control approach of Stimulated Depletion Quenching.This approach is applied to the phytochrome Cph8 bidirectional optogeneticswitch, and the results show significant improvement of its dynamic range.

Suppression of the Spectral Cross Talk of Optogenetic Switching by Stimulated Depletion Quenching. Theoretical Analysis