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Multi-photon microscopy is a powerful tool in biomolecular research. Lesscomplex and more cost effective excitation light sources will make thistechnique accessible to a broader community. Especially semiconductor diodeseeded fiber lasers have proven to be robust, low cost and easy to use.However, their wavelength tuning range is often limited, so only a limitednumber of fluorophores can be accessed. Therefore, different approaches havebeen proposed to extend the spectral coverage of these lasers. Recently, weshowed that four-wave mixing (FWM) assisted stimulated Raman scattering (SRS)can be harnessed to red-shift high power pulses from 1064 nm to a narrowbandoutput at 1122 nm and 1186 nm and therefore extend the number of accessiblefluorophores. In this contribution, we show the applicability of all threewavelengths for multi-photon microscopy and analyze the performance.

Wavelength agile multi-photon microscopy with a fiber amplified diode laser