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Photoacoustic spectral analysis is a novel tool for studying variousparameters affecting signals in Photoacoustic microscopy. But only observingfrequency components of photoacoustic signals doesn't make enough data for adesirable analysis. Thus a hybrid time-domain and frequency-domain analysisscheme has been proposed to investigate effects of various parameters likedepth of microscopy, laser focal spot size and contrast agent concentration onPhotoacoustic signals.

An Analysis Scheme for Investigation of Effects of Various Parameters on  Signals in Acoustic-Resolution Photoacoustic Microscopy of Mice Brain: a  Simulation Study