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Here, we report a facile method for electro-addressable functionalization ofa probe comprising of closely spaced three individually addressable carbonfiber electrodes for detection of nucleic acids. First, a multi electrode arrayprobe comprising three adjacent carbon fiber electrodes was fabricated throughpulling a three-barrel glass capillary with a single carbon fiber in eachbarrel using a micropuller. Second, electropolymerization based doping was usedfor electro-addressable functionalization of the multi-electrode array probe.To demonstrate that the current strategy works, anti-miR-34a was electrograftedon only one of three electrodes by electropolymerization of pyrrole on thespecific electrode. A second electrode was coated only with polypyrrole (PPy)and the third was left unmodified. Electrochemical impedance spectroscopy (EIS)was used for analysis and the results clearly showed charge transfer resistanceof the PPy + anti-miR-34a modified electrode increased significantly afterhybridization, while charge transfer resistance of the other two electrodesremained almost constant. The results demonstrate that the present strategy hasgreat potential for constructing multiplex nucleic acid micro/nano biosensorsfor local and in situ detection of multiple nucleic acid molecules such asmiRNAs at a time.

Using electropolymerization based doping for electro-addressable functionalization of a multi-electrode array probe for nucleic acid detection