English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

DNA and protein microarrays are a high-throughput technology that allow thesimultaneous quantification of tens of thousands of different biomolecularspecies. The mediocre sensitivity and dynamic range of traditional fluorescencemicroarrays compared to other techniques have been the technology's Achilles'Heel, and prevented their adoption for many biomedical and clinical diagnosticapplications. Previous work to enhance the sensitivity of microarray readout tothe single-molecule ('digital') regime have either required signal amplifyingchemistry or sacrificed throughput, nixing the platform's primary advantages.Here, we report the development of a digital microarray which extends both thesensitivity and dynamic range of microarrays by about three orders ofmagnitude. This technique uses functionalized gold nanorods as single-moleculelabels and an interferometric scanner which can rapidly enumerate individualnanorods by imaging them with a 10x objective lens. This approach does notrequire any chemical enhancement such as silver deposition, and scans arrayswith a throughput similar to commercial fluorescence devices. By combiningsingle-nanoparticle enumeration and ensemble measurements of spots when theparticles are very dense, this system achieves a dynamic range of about onemillion directly from a single scan.

A digital microarray using interferometric detection of plasmonic nanorod labels