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This paper reports the analytical method for gamma-amino butyric acid in thegerminated brown rice by liquid chromatography. GABA was derivatized bypre-column derivatization with 2, 4-dinitrofluorobenzene. To separate GABA inthe germinated brown rice extracts containing various amino acids, we reviewedthe influence of the kind of separation columns and the gradient of mobilephase of ultra performance liquid chromatography. GABA was extracted withdistilled water from the germinated brown rice with the optimum extraction timeof 12 h. To validate this method the precision and the recovery were discussed.In the concentration range from 5 to 50 microgram per mililiter, thecalibration curve for GABA was linear and the regression equation was obtainedwith correlation coefficient of 0.999. GABA was analyzed by amino acid analyzerin comparison with this method and the results of F-test and t-test indicatedthat there were no significant differences in accuracy and precision betweentwo methods.

Analysis of gamma-amino butyric acid in the germinated brown rice by pre-column derivatization with 2, 4-dinitrofluorobenzene