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The possibility of carbohydrate separation in BEH HILIC (Ethylene BridgedHybride, Hydrophilic Interaction Liquid Chromatography) column was studied byultra-performance liquid chromatography (UPLC) with evaporative lightscattering detector (ELSD) and mobile phase containing amine compounds asmodifiers. The chromatography conditions and ELSD parameters were optimized toseparate five typical carbohydrates and applied to analysis of four infant milkpowders. The linear ranges of carbohydrate determination were 20-300mg/L forfructose and glucose, 20-250mg/L for sucrose and lactose, and 35-180mg/L forfructo-oligosaccharide. The LODs were 16.4mg/L for fructose and glucose,17.3mg/L for sucrose, 20.0mg/L for lactose, and 46.7mg/L forfructo-oligosaccharide. Relative standard deviations (RSDs) ranged between3.45-4.23%, 1.46-4.17%, 4.14-5.60%, 1.39-4.09%, and 2.49-3.61% for fructose,glucose, sucrose, lactose, and fructo-oilgosaccharide, respectively andrecoveries ranged between 95.0 and 105.4%

Determination of carbohydrates in infant milk powders by  ultra-performance liquid chromatography with evaporative light scattering  detector and BEH HILIC column