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This work demonstrates the Nd:YAG waveguide laser as an efficient platformfor the bio-sensing. The waveguide was fabricated in the Nd:YAG crystal by thecooperation of the ultrafast laser writing and ion irradiation. As the laseroscillation in the Nd:YAG waveguide is ultra-sensitivity to the externalenvironment of the waveguide. Even a weak disturbance would induce a largevariation of the output power of the laser. According to this feature, theNd:YAG waveguide coated with Graphene and WSe2 layers is used as substrate forthe microfluidic channel. When the microflow crosses the Nd:YAG waveguide, thelaser oscillation in the waveguide is disturbed, and induces the fluctuation ofthe output laser. Through the analysis of the fluctuation, the concentration ofthe dextrose solution and the size of the tumor cell are distinguished

Ultrasensitive biosensor based on Nd:YAG waveguide laser: Tumor cell and Dextrose solution