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In response to cold stress, Drosophila Melanogaster increase their expressionof Frost, a candidate gene involved in cold response. The direct role of Frostin cold tolerance is yet to be determined, and its importance for survival incold environments has been questioned. In this study, I attempt to betterunderstand the molecular machinery of Frost by selecting for its protein in flylysate knowing only its RNA has been found. Detection of Frost expression andsubsequently studying its protein will hopefully lead to enhanced comprehensionof cold responses in insects, which is the long-term goal of this research. Ipredict that Frost will be expressed in flies that undergo cold stress at 0oCfor 2 hours before recovering at 220C for 3 hours before lysing. Two Westernblots were executed using fly lysate that underwent the above treatment, whoseantibodies were specific to Frost. No bands were seen in any of the lanescontaining treated samples in either of the Immunoblots, indicating that Frostwas either not expressed or not detected in fly lysate.

The Detection and Localization of Frost Protein in Drosophila