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Thiol self assembled monolayers (SAMs) are widely used in many nano- andbio-technology applications. We report a new approach to create andcharacterize a thiol SAMs micropattern with alternating charges on a flatgoldcoated substrate using atomic force microscopy (AFM) and Kelvin probe forcemicroscopy (KPFM). We produced SAMs patterns made of alternating positivelycharged, negatively charged, and hydrophobic terminated thiols by an automatedAFM assisted manipulation, or nanografting. We show that these thiol patternspossess only small topographical differences as revealed by AFM, anddistinguished differences in surface potential (20 to 50 mV), revealed by KPFM.The pattern can be helpful in the development of biosensor technologies,specifically for selective binding of biomolecules based on charge andhydrophobicity, and serve as a model for creating surfaces with quantifiedalternating surface potential distribution.

AFM-Assisted Fabrication of Thiol SAM Pattern with Alternating Quantified Surface Potential