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The aims of the current study were to establish a system of culture forinduction of paralysed chondrocytes and to investigate if these cells arereally dying. Chondrocytes were isolated from the growth cartilage of fetalequines, centrifuged and cultured as pellets in either 10% fetal calf serum or10% horse serum for 28 days and processed for light and electron microscopy.Different cell types were counted and expressed as a percentage to the totalcell number. Growth kinetics including the pellet weight and thickness and thecellular density were evaluated. After 7 days in culture, paralysedchondrocytes with similar morphology to those described in the rabbit growthcartilage could be identified in pellets in each serum type, however, theproportion of the cells was different. In pellet cultured with 10% fetal calfserum, more than 50% of the cells were paralysed chondrocytes but in 10% horseserum, less than 10% of cells were of paralysed type. At day 14, about 50% ofthe cells in pellets cultured in either serum type differentiated intohypertrophic dark chondrocytes and the proportion of paralysed cells wasmarkedly decreased. After 21 days in each culture, more than 70% of the cellswere hypertrophic dark chondrocytes and no paralysed chondrocytes could beobserved. The paralysed chondrocytes may be not dying and they likely to be animmature form of hypertrophic dark chondrocytes. It is better to use the termimmature dark chondrocytes instead of paralysed cells. This culture system willbe useful for further molecular studies on paralysed chondrocytes and toexplore the functions of these cells.

Are Paralysed Chondrocytes Really Dying?