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In this article we present a new diagnostic approach utilizing flow-cytometryto study compounds of nanoparticle samples in solution by analysis of theirscattering patterns retrieved from the cytometric measurements. As a specificenhancement of this technique we study as well the scattering pattern ofnanoparticles in a fluorescent solution (529 nm). A significant enhancement ofthe cytometry measurements is observed supporting an improved separation ofparticle formations that are clearly resolved in the cytograms. The samples inthis experiment are prepared from 80 nm citrate-capped gold nanoparticles(AuNP). They are stabilized providing 2-(dimethylamino)ethanol (DMAE) in theaqueous solution. A laser diode with a wavelength of 488 nm is used asfundamental illumination for the flow-cytometry measurements (FCM). DynamicLight Scattering (DLS) measurements are performed separately and demonstrate avery good agreement with the Flow-Cytometry measurements both of which allow togive an effective size calibration. For further analysis light transportsimulations are presented. They provide information on the key-process to formthe correlation of the fluorescent solutions FCM to the studied particles ofinterest. From this we extract the volume nature of the scattering processensuring the correlation.

Investigations of dynamic light scattering properties in fluorescent solution: A comprehensive study using plasmonic enhanced Flow-Cytometry